Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for microbiological sample collection from food processing equipment and other environmental surfaces where neutralization of residues of disinfectants is required which have antimicrobial activity.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 10.000 |
| HM peptone B # | 5.000 |
| Lecithin | 0.700 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
- To label write on the white print area of the sampling bag.
- Tear off the top of the bag along the tear line and pull the tabs to open the bag.
- Guide the sponge stick with handle towards the top and remove the sponge stick bygrasping the handle without touching the internal surfaces of the bag.
- Sample the area by ensuring the entire sponge makes full contact with the surface.
- Transfer the sponge stick inside the bag halfway and hold sponge in place inside bag. Snapoff the sponge from the sponge stick, discard the handle while the sponge stays inside the bag.
- By holding the taped wires roll the bag appropriately and close the bag by folding the taped wires.
- Incubate the sampled bag at 35-37°C for 18-24hours.
Principle And Interpretation
Letheen Broth was developed by Quisno, Gibby and Foter (1) by the addition of lecithin and Polysorbate 80 to FDA Broth. Letheen Broth is recommended by AOAC to determine the phenol coefficient of cationic surfactants (2). Letheen Medium is also recommended for testing of cosmetics (3). HM peptone B, Peptone, supply essential nutrients and other trace elements for the microbial growth. Lecithin and polysorbate 80 enables the recovery of bacteria from solutions containing residues of disinfectant used in sanitization of utensils and equipments. Lecithin neutralizes quaternary ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene and formalin (4).
Type of specimen
Environmental samples, food industry samples
Specimen Collection and Handling
For enviromental samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (5,6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves / protective clothing / eye protection / face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement or observation.
- Individual strain of a microorganism may have unique growth requirements with respect to nutrients and physical conditions. Based on which the growth pattern of each varies on a medium and some even may display significant delay.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality control
Appearance
Sterile HiEnviro-Sponge™ Stick Hydrated w/10ml Letheen Broth, in wired sampling bag
Dose of Irradiation (kGy)
> 25kGy
Sterility check
Passes release criteria
Cultural Response
Check for recovery of low CFU Organisms (~10 CFU/ml), inoculated on hydrated sponge w/ 10ml Letheen Broth with hold
time of 1 hr ± 15 mins at 20-25°C. Further enrichment in Soyabean Casein Digest Medium is carried out with incubation at
30-35°C for 18-24 hrs, followed by recovery on Selective media.
| Organism | Growth |
|---|---|
| Escherichia coli ATCC 25922 (00013*) |
good-luxuriant (Recovery on MacConkey Agar, MP081) |
| Salmonella Typhimurium ATCC 14028 (00031*) |
good-luxuriant (Recovery on XLD Agar, MP0311) |
| Listeria monocytogenes ATCC 19111 (00020*) |
good-luxuriant (Recovery on HiCrome™ Listeria Ottaviani-Agosti Agar Base, MP1540I) |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf life
Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | HiEnviro-Sponge™ Stick Hydrated w/10ml Letheen Broth, sterile |
|---|---|
| SKU | EST002 |
| Customized Product Available | No |
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