Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for microbiological sample collection from food processing equipment and other environmental surfaces.
Composition
| Ingredients | g/L |
|---|---|
| Peptone # | 10.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate dodecahydrate | 9.000 |
| Potassium dihydrogen phosphate | 1.500 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Enzymatic digest of casein
Directions
- A white printed area has been provided to label the sample.
- Tear the bag along the tear-line and pull the tabs to open the bag.
- Guide the sponge out of the bag without touching the bag's internal surface (wearing sterile hand gloves is mandatory while getting the sponge out).
- Swab the surface ensuring that the entire sponge is in contact with the surface of the collection site.
- Place the sponge back in the bag.
- Roll the top opening of the sampling bag by holding the tapped wires. Seal the bag by folding the tapped wires at both ends.
- Incubate the sampled bag at 35-37°C for 18-24hours.
Principle And Interpretation
Microorganisms that are subjected to environmental stresses may become structurally or metabolically damaged or injured. These microorganisms are unable to replicate in selective environments. Therefore, these injured organisms must be resuscitated or permitted to repair the damage by incubation in an appropriate, non-selective environment. Edel and Kampelmacher (1) noted that sub-lethal injury to Salmonellae may occur in many food preservation processes. Enriching injured cells in Lactose Broth (pH 6.9) may be further detrimental to their recovery (2). Pre-enrichment in Buffered Peptone Water at 35°C for 18-24 hours results in repair of injured cells (3). The buffering system prevents bacterial damage due to change in the pH of the medium. Recently ISO committee has also recommended this pre-enrichment medium for the detection of Enterobacteriaceae from food stuffs and animal feeding stuffs, water, milk, milk products and other products (4).
Type of specimen
Environmental samples, food industry samples
Specimen Collection and Handling
For enviromental samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (5,6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves / protective clothing / eye protection / face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement or observation.
- Individual strain of a microorganism may have unique growth requirements with respect to nutrients and physical conditions. Based on which the growth pattern of each varies on a medium and some even may display significant delay.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality control
Appearance
Sterile HiEnviro-Sponge™ (Cellulose sponge) Hydrated w/ 10ml Buffered Peptone water in
wired sampling bag
Dose of Irradiation (kGy)
> 25 kGy
Sterility check
Passes release criteria
Cultural Response
Check for recovery of low CFU Organisms (~10 CFU/ml), inoculated on hydrated
sponge w/ 10ml Buffered Peptone water with hold time of 1 hr ± 15 mins at 20-25°C. Further enrichment in
Soyabean Casein Digest Medium (is carried out with incubation at 30-35°C for 18-24 hrs, followed by recovery
on Selective media.
| Organism | Growth |
|---|---|
| Escherichia coli ATCC 25922 (00013*) |
good-luxuriant (Recovery on MacConkey Agar, MP081) |
| Salmonella Typhimurium ATCC 14028 (00031*) |
good-luxuriant (Recovery on XLD Agar, MP0311) |
| Listeria monocytogenes ATCC 19111 (00020*) |
good-luxuriant (Recovery on HiCrome™ Listeria Ottaviani-Agosti Agar Base, MP1540I) |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf life
Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | HiEnviro-Sponge™ Hydrated w/10ml Buffered Peptone water, sterile |
|---|---|
| SKU | ESM001 |
| Customized Product Available | No |
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