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Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 20.000 |
| Yeast extract | 10.000 |
| Dextrose | 20.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Each capsule contains 16.25 gms of dehydarted medium. Suspend 1 capsule in 250 ml (4 capsules in 1000 ml) distilled or purified water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Principle And Interpretation
YPD (YEPD) Growth Agar is used for the growth of Saccharomyces cerevisiae. Yeasts are unicellular eukaryotes and extensively studied model organism in molecular genetics. They are chemoorganotrophs as they utilize organic compounds as a source of energy. YPD (YEPD) Growth Agar is used for the maintenance and propagation of yeasts including S. cerevisiae in various molecular microbiology procedures (1, 2). YPD functions as a complete medium for yeast growth and it contains yeast extract, peptone and glucose or dextrose. Yeast extract supplies B-complex vitamins and it contains all the amino acids necessary for growth. Peptone acts as the source of nitrogen, vitamins and minerals. Dextrose serves as the carbon source. This medium supports the vigorous growth of wild type as well as mutant strains of all kinds of budding yeast.
Type of specimen :
Not applicable, Wild and mutant strains of Saccharomyces cerevesiae under study.
Specimen Collection and Handling:
Follow appropriate laboratory techniques for handling culture, processing as per guidelines and local standards (3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Being a non selective medium, other heterotrophs may also grow on this medium. It cannot be used as a selection medium exclusively for auxotrophy studies.
Performance and Evaluation :
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Gelatin capsule containing cream to yellow coloured granular media
Prepared Medium
Amber clear to slight opalescent gel forms in Petri plates.
Quantity
Each capsule contains 16.25 grams of medium sufficient for 250 ml media
pH
6.30-6.70
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | good-luxuriant |
Key = * Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | HiEncap™ YPD Growth Agar (HiEncap™ YEPD Growth Agar) |
|---|---|
| SKU | ECG038CCL |
| Product Type | HiEncap™ |
| Physical Form | Granulated Media in Gelatin Capsule |
| Origin | Animal |
| Packaging type | HDPE Jar |
| References | 1. Adams, A., D. E. Gottschling, C. A. Kaiser, and T. Stearns. 1997.Methods in yeast genetics: A Cold Spring Harbor Laboratory Course Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 2. Burke, D., Dawson, D.,and T. Stearns. 2000. Method in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. |
| Customized Product Available | No |
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YPD (YEPD) Growth Agar
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