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HiEncap™ SOC Growth Medium
A special medium for competent cells to allow expression of transferred resistance genes before exposing cells to selective conditions.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 20.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 0.500 |
| Magnesium sulphate, hepta hydrate | 5.000 |
| Glucose | 3.600 |
**Formula adjusted, standardized to suit performance parameters
Directions
Each capsule contains 15.77 gms of dehydrated medium. Suspend 500ml (2 capsules in 1000 ml) distilled or purified water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
SOC Growth Medium is a special medium for incubating competent cells immediately after transformation to allow expression of transferred resistance genes before exposing cells to selective conditions (1). E. coli cells are first made competent during transformation where perforations are made in the bacterial cells so that the foreign DNA can penetrate the cells. To repair cells from this damage SOC medium is used. This medium is same as SOB medium except glucose is added as the carbon source which repairs the perforations of the E. coli cells. Furthermore, the transferred resistance genes are expressed in this medium before exposure to selective conditions (1). Tryptone provides nitrogen, amino acids and other growth factors which permit the cells to go through the stress of transformation. Vitamins and trace elements are contained in Yeast Extract. Sodium chloride provides essential ions for transport and osmotic balance. Magnesium sulfate provides magnesium ions which are required in a variety of enzymatic reactions, including DNA replication.
Type of specimen
Not applicable, Recombinant Escherichia coli culture under study.
Specimen Collection and Handling
Follow appropriate laboratory techniques for handling culture, processing as per guidelines and local standards (1). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- If the cells are not grown to desired competency required in the medium, transformation may not be successful.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Gelatin capsule containing cream to yellow coloured granular media
Prepared Medium: Light yellow clear solution without any precipitate
Quantity: Each capsule contains 15.77 grams of medium sufficient for 500 ml media
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) |
Growth |
|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | good-luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good-luxuriant |
| Escherichia coli DH5 alpha МТСС 1652 | 50-100 | good-luxuriant |
Key = * Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | HiEncap™ SOC Growth Medium |
|---|---|
| SKU | ECG015D |
| Product Type | HiEncap™ |
| Physical Form | Granulated Media in Gelatin Capsule |
| Origin | Animal |
| Packaging type | HDPE Jar |
| References | 1. Sambrook J., E. F. Fritsch, and T. Maniatis. 198 |
| Customized Product Available | No |
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