Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for molecular biology use.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 20.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 0.500 |
| Magnesium sulphate | 2.400 |
| Potassium chloride | 0.186 |
| Final pH (at 25°C) | 7.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Each capsule contains 7.02 gms of medium. Suspend 1 capsule in 250 ml (4 capsules in 1000ml) distilled or purified water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 20% v/v glucose solution (i.e. 5 ml to 250ml or 20 ml to 1000ml media). Mix well and dispense into sterile tubes or flasks as desired.
Principle And Interpretation
SOC Broth Base is a medium which is prepared by adding 20% glucose solution to SOB Medium (Hanahans Broth) (M1252). This medium is a nutritionally rich growth medium used for growing bacterial cells, for preparing chemically competent cells and in the recovery step of competent cell transformations. E. coli is first grown in SOB Medium (Hanahans Broth) (M1252) to get the desired cell density. The cells are then harvested and subjected to chemical treatment or electoporation to develop competent cells. These competent cells are then transformed using suitable method. The transformants are then grown in SOC Medium. The use of SOC Broth maximizes the transformation efficiency of competent cells (3).
Tryptone and yeast extract serve as rich sources of nitrogen and growth factors which are readily available to the bacteria that are under stress due to transformation procedures. These sources of nutrients allow them to recover from stress and grow well. Potassium and sodium chloride maintain isotonic conditions. Magnesium sulphate is a source of magnesium ions required in a variety of enzymatic reactions including DNA replication.
Type of specimen
Isolated Recombinant Escherichia coli
Specimen Collection and Handling
For isolated microorganism samples follow appropriate techniques for handling specimens as per established guidelines (3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- This medium is general purpose medium and may not support the growth of fastidious organisms.
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Gelatin capsule containing cream to yellow coloured granular media
Colour and Clarity of prepared medium: Light amber coloured clear solution without any precipitate forms in tubes
Quantity: Each capsule contains 7.02 grams of medium sufficient for 250 ml media
Reaction: Reaction of 2.8% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH: 6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli DH5 alpha MTCC 1652 | 50-100 | luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | HiEncap™ SOC Broth Base |
|---|---|
| SKU | EC1379CCL |
| Product Type | HiEncap™ |
| Physical Form | Granulated Media in Gelatin Capsule |
| Origin | Animal |
| Packaging type | HDPE Jar |
| References | 1. Sambrook J., Fritsch E. E. and Maniatis T., 1989, Molecular Cloning : A Laboratory Manual, 2nd Ed., Cold Spring Harbor Lab., Cold Spring Harbor, N.Y. |
| Customized Product Available | No |
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