Glutathione Agarose Resin

Glutathione agarose Resin contains glutathione, immobilized on agarose and is used for rapid purification of glutathione S-transferase (GST) or glutathione binding proteins. For the purification of recombinant proteins additional amino acids or a whole protein is often added and is known as fusion tags. One of the most common fusion tag is glutathione S-transferase (GST) which can be added to a protein of interest to purify it. GST protein has a strong binding affinity for glutathione, immobilized on agarose, and when a mixture of proteins is added to this matrix, the protein of interest (tagged to GST) binds to the glutathione ligand and impurities are removed by washing with binding buffer. As a result the protein is isolated from the rest. Finally, the beads are treated with reduced glutathione (free) which competitively detaches the interaction between immobilized glutathione and the GST-tagged protein of interest from the beads which results in a purified protein