Protein A Agarose Resin

HiMedia’s Protein A Agarose Resin consists of rProtein A covalently bound to crosslinked agarose beads and has a very high affinity for IgG (immunoglobulins). rProtein A is a recombinant protein which contains five homologous antibody–binding domains with high affinity for subclasses of IgG from many species e.g. human, rabbit, mouse, sheep etc. and for some IgA and IgM. Protein A is covalently coupled to agarose to prepare an affinity matrix for isolating IgGs from various species. As it binds specifically to the Fc region of IgG molecules it is used for the f following: Purification of IgG fractions (from crude serum, ascites fluid, tissue culture supernatant). Isolation of antigen-antibody complex (during immunoprecipitation). This resin is used for batch or column purifications of classes, subclasses and fragments of immunoglobulins