HiSep™ LSM 1073

It is based on the adapted method of isolating lower density mononuclear cells using centrifugation techniques by Bøyum in which defibrinated blood is layered on a solution of sodium diatrizoate and polysucrose and centrifuged at low speeds for 30 minutes. Differential migration following centrifugation results in the formation of several cell layers. Mononuclear cells(monocytes or mesenchymal stromal cells) are contained in the banded plasma-LSM interphase due to their density, and the pellet that is formed contains mostly higher density lymphocyte, erythrocytes and granulocytes, which have migrated through the gradient to the bottom of the tube. Mononuclear cells are recovered by aspirating the plasma layer and then removing the cells. HiSepTM LSM 1073 is a solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.073± 0.0010g/ml. This medium offers a quick and reliable method for the simple isolation of lower density mononuclear cells from human peripheral blood, umbilical cord blood and bone marrow.