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Buffered Sodium Chloride Peptone Solution w/3% Polysorbate 80 and 0.3% Soya Lecithin

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As low as ₹10,296.00
Availability: In stock
Only %1 left
SKU:
LQ566D

Intended use

For the preparation of test suspension.

Composition**

Ingredients g/L
HMC Peptone# 1.000
Potassium dihydrogen phosphate 3.600
Disodium hydrogen phosphate dihydrate 7.200
Sodium chloride 4.300
Polysorbate 80 (Tween 80) 30.00
Lecithin 3.000
Final pH (at 25°C) 7.00 ±0.5

# Equivalent to Peptone (meat or casein)
** Formula adjusted, standardized to suit performance parameters

Directions -

Label the ready to use LQ566D bottle. Inoculate the sample and Incubate at specified temperature and time.

Principle And Interpretation

The composition of Buffered Sodium Chloride Peptone Solution is in accordance with the harmonized methodology of USP/EP/BP/JP/IP (1-5). This medium is recommended for preparation of stable test strain suspension employed for validating the microbiological testing procedures of non-sterile products. The standardized stable suspensions are used so that the suitability of this test to detect microorganism in presence of product can be established. Non-fatty products insoluble in water and water-soluble products are diluted/dissolved using this solution. HMC Peptone serves as nutrient source and maintains the cell viability. Phosphates in the medium act as good buffering agents. Sodium chloride maintains the osmotic balance. Addition of Lecithin and polysorbate 80 (Tween 80) are reported to inactivate residual disinfectants from where the sample is collected (6). Lecithin neutralizes quaternary ammonium compounds, Polysorbates reduce surface tension and also inactivate phenolic compound, hexachlorophene, formalin and with lecithin ethanol (7), if present in the test sample.

Type of specimen

Pharmaceutical samples

Specimen Collection and Handling

For pharmaceutical samples follow appropriate techniques for handling specimens as per established guidelines (1-5). After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions

Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations

  1. This medium contains less nutrients and is not recommended for the growth of microorganisms.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance: Sterile clear Buffered Sodium Chloride Peptone Solution w/3% Polysorbate 80 and 0.3% Soya lecithin in a glass bottle.

Colour: Light yellow coloured opalescent solution

Quantity of medium: 500ml of medium in bottle

pH: 6.50-7.50

Sterility Check: Passes release criteria

Cultural response:

Cultural characteristics observed after recovery on Soybean Casein Digest Agar after an incubation at 30-35°C for 18-24 hours for bacteria and Sabouraud Dextrose Agar at 30-35°C for 24-48 hours.

Organism Inoculum
(CFU)		 Recovery
within 2 hours
of incubation		 Recovery
within 4 hours
of incubation		 Recovery
within 24 hours
of incubation
Escherichia coli ATCC
8739 (00012*)		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)
Escherichia coli ATCC
25922 (00013*)		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)
Staphylococcus aureus
subsp. aureus ATCC
6538 (00032*)		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)
Staphylococcus aureus
subsp. aureus ATCC
25923 (00034*)		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)
^Pseudomonas
paraeruginosa ATCC
9027 (00026*)		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)
^Pseudomonas
paraeruginosa ATCC
27853 (00025*)		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)
Salmonella Typhimurium
ATCC 14028 (00031*)		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)
Salmonella Abony NCTC
6017 (00029*)		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)
**Bacillus spizizennii
ATCC 6633 (00003*)		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)
$ Kokuria rhizophila
ATCC 9341		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)
Candida albicans ATCC
10231 (00054*)		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)
Candida albicans ATCC
2091 (00055*)		 50-100 no decrease in
colony count		 no decrease in
colony count		 no decrease in
colony count
(stored at
2-8°C)

Key: (*) Corresponding WDCM numbers, $ Formerly known as Micrococcus luteus
^ Formerly known as Pseudomonas aeruginosa * Formerly known as Bacillus subtilis subsp. spizizenii

Storage and Shelf Life

Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).

More Information
Product Name Buffered Sodium Chloride Peptone Solution w/3% Polysorbate 80 and 0.3% Soya Lecithin
SKU LQ566D
Customized Product Available No
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