Dulbecco’s Modified Eagle Medium (DMEM) w/ L-Glutamine and Sodium pyruvate w/o Glucose and Sodium bicarbonate

Dulbecco's Modified Eagle Medium is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs glucose per litre and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4.5gms/L of glucose. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. AT186 is Dulbecco's Modified Eagle Medium with Lglutamine and Sodium pyruvate. It does not contain Glucose and sodium bicarbonate.