Leibovitz’s L-15 Medium w/ L-Glutamine w/o Sodium Pyruvate and Sodium bicarbonate

Leibovitz's Medium was specifically designed to grow cells in a CO2 free atmosphere. The standard sodium bicarbonate/CO2 buffering system is replaced by combination of free basic amino acids, phosphate buffers and higher levels of galactose and sodium pyruvate. As a result, the medium does not require supplementation with sodium bicarbonate and can be used under conditions of free gaseous exchange with the atmosphere. The medium can be used to grow human tumor cells and embryonic cells and also established cell lines like HeLa and Hep-2. The medium is frequently used in diagnostic virology where tissue cell lines or strains need to be grown in closed systems. Leibovitz's medium obviates the need of frequent medium change. AT164 is Leibovitz's Medium with L-glutamine. It does not contain sodium pyruvate.