Dulbecco's Modified Eagle Medium (DMEM), Low glucose w/ 1gm Glucose per litre, L-Glutamine, 25mM HEPES buffer and Sodium Pyruvate w/o Sodium bicarbonate

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of Glucose and was originally used to culture embryonic mouse cells. AT149 is Dulbecco's Modified Eagle Medium and contains 1gm glucose per litre, L-glutamine, sodium pyruvate and 25mM HEPES buffer. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.