Dulbecco’s Modified Eagle Medium (DMEM), Low glucose (Modified for Autoclaving) w/ 1gm Glucose per litre and Sodium pyruvate w/o L-Glutamine and Sodium bicarbonate

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of Glucose and was originally used to culture embryonic mouse cells. AT065A is DMEM low glucose and is modified for autoclaving. Autoclavable medium offers a convenient alternative to membrane sterilized liquid medium and is modified to include heat stable components to ensure that product efficacy is maintained after autoclaving. L-glutamine is heat labile, hence has been omitted from the formulation.