Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Antibiotic Assay Medium C
As the broth medium in turbidimetric assay of a wide variety of antibiotics in accordance with EP/BP.
Gms/Lit : 19.90
25.13 Lit/500G
Gms/Lit : 19.90
25.13 Lit/500G
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Intended Use
Used as the broth medium in turbidimetric assay of a wide variety of antibiotics in accordance with EP/BP.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 6.000 |
| HM peptone B # | 1.500 |
| Yeast extract | 3.000 |
| Sodium chloride | 3.500 |
| Glucose monohydrate | 1.000 |
| Dipotassium hydrogen phosphate | 3.680 |
| Potassium dihydrogen phosphate | 1.320 |
| pH after sterilization | * 7.0±0.1 |
Formula adjusted, standardized to suit performance parameters
*While assaying Josamycin & Josamycin propionate adjust the pH to 8.0 ±0.1
# Equivalent to Beef extract
Directions
Suspend 19.9 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml R-water/purified/distilled water. Heat with frequent agitation to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Adjust the pH of the medium, using freshly prepared buffer solution as recommended by the EP for the antibiotic assayed.
Note: Adjust the medium pH to 8.0 ± 0.1 for assaying Josamycin & Josamycin propionate.
Advice: Recommended for the Microbiological assay of Colistimethate sodium, Colistin sulfate, Framycetin sulphate, Gentamicin sulphate, Gramicidin, Kanamycin monosulphate, Kanamycin acid sulphate, Neomycin sulphate, Rifamycin sodium, Spiramycin, Streptomycin sulphate, Tylosin, Tylosin tartrate, Tyrothricin and Vancomycin hydrochloride in accordance with E.P.
Principle And Interpretation
This medium is used in turbidimetic assay of several antibiotics. The composition of the medium is in accordance to the specifications detailed in the European Pharmacopoeia and British Pharmacopoeia (1,2). Turbidimetric methods for determining the potency of antibiotics are inherently more accurate and more precise than comparable agar diffusion procedures (3).
Peptone, HM peptone B and yeast extract provide essential nutrients and growth factors for enhanced microbial growth. Sodium chloride maintains the osmotic equilibrium while phosphates are incorporated in the medium to provide good buffering action. Glucose monohydrate serves as the carbon and energy source for faster growth.
Turbidimetric antibiotic assay is based on the change or inhibition of growth of a test microorganisms in a liquid medium containing a uniform concentration of an antibiotic (4). Use of this method is appropriate only when test samples are clear.
Type of specimen
Antibiotics as per EP and BP
Specimen Collection and Handling
Follow appropriate techniques for handling specimens as per established guidelines (1,2).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection.
Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Freshly prepared plates should be used for antibiotic assays.
- Use of this method is appropriate only when test samples are clear.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured clear solution without any precipitate
pH: 6.90-7.10
Growth promotion test: In accordance with EP/BP
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) |
Growth | Serial dilution with |
|---|---|---|---|
| Escherichia coli ATCC 9637 | 50-100 | luxuriant | Colistimethate sodium, Colistin sulfate |
| Escherichia coli ATCC 10536 | 50-100 | luxuriant | Rifamycin sodium |
| Enterococcus hirae ATCC 10541 (00011*) | 50-100 | luxuriant | Gramicidin, Tyrothricin |
| Klebsiella pneumoniae ATCC 10031 | 50-100 | luxuriant | Streptomycin sulphate |
| Staphylococcus aureus ATCC 6538P | 50-100 | luxuriant | Framycetin sulphate, Genatamicin sulphate, Gramicidin, Kanamycin monosulphate, Kanamycin acid sulphate, Neomycin sulphate, Spiramycin; For Josamycin & Josamycin propionate-adjust the pH of the medium to 8.0 ± 0.1, For Vancomycin hydrochloride incubate at 37-39°C. |
| Staphylococcus aureus ATCC 9144 (00035*) | 50-100 | luxuriant | Tylosin, Tylosin tartarate |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and use freshly prepared medium. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
| Product Name | Antibiotic Assay Medium C |
|---|---|
| SKU | MAP555 |
| Customized Product Available | No |
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