Iscove’s Modified Dulbecco’s Medium w/ L-Glutamine, 25mM HEPES buffer and Sodium bicarbonate w/o Glucose

Iscove's Modified Dulbecco's Medium(IMDM) is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium contains additional amino acids, sodium selenite, sodium pyruvate, vitamins and inorganic salts. Potassium nitrate is substituted by ferric nitrate. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. AL230A contains L-glutamine, 25mM HEPES buffer and sodium bicarbonate. It does not contain glucose and sodium bicarbonate.