Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/ 4.5gms Glucose per litre, Sodium bicarbonate and Sodium pyruvate w/o L-Glutamine

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4500mgs/L of glucose. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. AL007 is Dulbecco's Modified Eagle Medium with 4.5gms glucose per litre, sodium pyruvate and sodium bicarbonate. It does not contain L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiologica growth requirements specific for different cell lines.