Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Agar Medium C (Sabouraud-Glucose Agar with Chloramphenicol)
For selective cultivation of yeasts and moulds in accordance with EP/BP/IP.
Gms/Lit : 61.41
8.14 Lit/500G
8.14 Lit/500G
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Intended Use:
Recommended for selective cultivation of yeasts and moulds in accordance with EP/BP/IP.
Composition**
| Ingredients | g/L |
|---|---|
| HMC peptone # | 10.000 |
| Dextrose monohydrate (Glucose monohydrate) | 40.000 |
| Chloramphenicol | 0.050 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Peptones (meat and casein)
Directions
Suspend 61.41 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes or as per validated cycle. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Sabouraud Glucose Agar Medium with Chloramphenicol is cited as Medium C and recommended for cultivation of yeasts and moulds by EP/BP/IP (1,2,3). This medium was described originally by Sabouraud (2) for the cultivation of fungi, particularly useful for the fungi associated with skin infections. The medium is often used with antibiotics such as Chloramphenicol (4) for the isolation of pathogenic fungi from materials containing large numbers of fungi or bacteria.
HMC peptone provide nitrogenous compounds. Glucose monohydrate provides an energy source. Chloramphenicol inhibits a wide range of Gram-positive and Gram-negative bacteria, which makes the medium selective for fungi (5). The low pH favours fungal growth and inhibits contaminating bacteria from clinical specimens (6). Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet.
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling
For pharmaceutical samples follow appropriate techniques for handling specimens as per established guidelines (1,2,3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Certain pathogenic fungi may show poor growth on this medium.
- Overheating of the medium may result in low productivity and softening of gel.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in Petri plates
pH: 5.40-5.80
Growth Promotion Test
Cultural response was observed in accordance with EP, BP, IP, after an incubation at 20-25 °C for <=5 days. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar and fungus growth on Sabouraud Dextrose Agar
Cultural Response
| Organism | Inoculum (CFU) | Growth | Observed Lot value (CFU) | Recovery | Incubation period |
|---|---|---|---|---|---|
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant (white colonies) | 25-100 | >=50% | <=5 d |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | 25-100 | >=50% | <=5 d |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant | 25-100 | >=50% | <=5 d |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | luxuriant | 35-100 | >=50% | <=5 d |
| Escherichia coli ATCC 25922 (00013*) | >=103 | inhibited | 0 | 0% | <=5 d |
| Escherichia coli ATCC 8739 (00012*) | >=103 | inhibited | 0 | 0% | <=5 d |
| Trichophyton rubrum ATCC 28191 | 50-100 | good | <=5 d | ||
| $Lactobacillus paracasei ATCC 334 | >=103 | inhibited | 0 | 0% | <=5 d |
Key: (*) Corresponding WDCM numbers,
(#) - Formely known as Aspergillus niger
($) - Formely known as Lactobacillus casei
Storage and Shelf Life
Store between 15-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Agar Medium C (Sabouraud-Glucose Agar with Chloramphenicol) |
|---|---|
| SKU | MAP1067 |
| Customized Product Available | No |
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