1M DTT

The use of dithiothreitol (DTT) was popularized by William Wallace Cleland who first published on its reducing potential in 1964. This led to dithiothreitol being referred to as Cleland's reagent. Dithiothreitol is a commonly used reducing agent and is soluble in a range of solvents including water, ethanol, acetone and ethyl acetate. DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro. DTT has a reducing potential of -0.33 V at pH 7. HiMedia’s 1M DTT is a sterile-filtered, pre-mixed solution. This is commonly used as an reducing agent and many other Molecular Biology applications. It is free of DNase and RNase.