Mollicutes includes over 100 species of Mycoplasma and the very closely related genus, Acholeplasma. The most prevalent Mycoplasma contaminants of cultured cells are Mycoplasma orale, Mycoplasma hyorhinis, Mycoplasma arginini, Mycoplasma fermentans and Mycoplasma hominis . Among bacterial infections, Mycoplasma contamination poses a particular challenge because it does not conspicuously overgrow the cell culture, can only be detected using special assays, and is resistant to many commonly used antibiotics. Hi-PCR® Mycoplasma SYBr PCR Kit represents a sensitive, specific, accurate, inexpensive and quick mycoplasma detection assay that is suitable for the routine screening of cell cultures.