Sakazakii DHL Agar is used for the detection and isolation of pathogenic Enterobacteriacae from all types of specimens.Sakazakii DHL Agar stands for Sakazakii Deoxycholate-Hydrogen sulphide-Lactose Agar. Sodium Deoxycholate in the medium is a selective agent and inhibits gram-positive bacteria and also prevents swarming growth of Proteus species. Lactose and sucrose are fermentable carbohydrate source. Sulphur is released from thiosulphate or other sulphur-containing compounds in the form of sulphide. The H2S thus produced is detected by ferric ammonium citrate to form insoluble heavy metal sulphides that appear as a black precipitate.