HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended Use
Actinomyces HiVeg Agar / Broth is recommended for the cultivation and maintenance of the anaerobic Actinomyces species.
Composition
| Ingredients | MV341 Grams/Litre |
MV233 Grams/Litre |
|---|---|---|
| HiVeg infusion | 10.00 | 10.00 |
| HiVeg hydrolysate No. 1 | 10.00 | 10.00 |
| HiVeg hydrolysate | 4.00 | 4.00 |
| Yeast extract | 5.00 | 5.00 |
| Dextrose | 5.00 | 5.00 |
| L-Cysteine hydrochloride | 1.00 | 1.00 |
| Starch, soluble | 1.00 | 1.00 |
| Sodium chloride | 5.00 | 5.00 |
| Monopotassium phosphate | 15.00 | 15.00 |
| Ammonium sulphate | 1.00 | 1.00 |
| Magnesium sulphate | 0.20 | 0.20 |
| Calcium chloride | 0.02 | 0.02 |
| Agar | 20.00 | - |
| Final pH (at 25°C) | 6.9 ± 0.2 | 7.2 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Product Profile
Vegetable based (Code MV)
| MV341/MV233 |
|---|
| HiVeg infusion |
| HiVeg hydrolysate |
| HiVeg hydrolysate No. 1 |
Animal based (Code M)
| M341/M233 |
|---|
| Beef heart infusion |
| Casein enzymic hydrolysate |
| Tryptose |
Recommended for
Cultivation and maintenance of anaerobic Actinomyces species
Reconstitution
(MV341): 77.22 g/l
(MV233): 57.22 g/l
Quantity on preparation (500g)
(MV341): 6.47 L
(MV233): 8.73 L
pH (25°C)
(MV341): 6.9 ± 0.2
(MV233): 7.2 ± 0.2
Supplement
None
Sterilization
121°C / 15 minutes.
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 77.22 grams of MV341 or 57.22 grams of MV233 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Distribute into tubes or flasks. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Actinomyces HiVeg Agar / Broth is prepared by using vegetable based peptones instead of animal based peptones making the medium BSE/TSE risk free. These media are the modifications of Actinomyces Agar/Broth which was formulated as a maintenance medium by Pine and Watson (1). The broth medium was further modified and recommended for growing and maintaining members of the genus Actinomyces (2).
HiVeg infusion, HiVeg hydrolysate, yeast extract, starch, dextrose and L-Cysteine provide carbon, nitrogen, sulphur, vitamins and other growth factors. Metallic salts provides essential electrolytes and minerals.
Quality Control
Appearance of Powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 2.0% Agar gel of MV341.
Colour and Clarity
Light amber coloured slightly opalescent gel with a slight precipitate forms in petri plates, clear solution with a slight precipitate in tubes.
Reaction
Reaction of 7.72% w/v aqueous solution of MV341 is pH 6.9 ± 0.2 at 25°C.
Reaction of 5.72% w/v aqueous solution of MV233 is pH 7.2 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 30°C for 18-48 hours.
| Organisms (ATCC) | Inoculum (CFU) |
Growth | Recovery |
|---|---|---|---|
| Streptomyces achromogenes (12767) | 102 | good | >50% |
| Streptomyces albus (3004) | 102 | good | >50% |
| Streptomyces lavendulae (8664) | 102 | good | >50% |
| Actinomyces israelii (10049) | <103 | luxuriant | >70% |
| Actinomyces bovis (13683) | <103 | good | >50% |
| Product Name | Actinomyces HiVeg™ Broth |
|---|---|
| SKU | MV233 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and McCartney, Practical Medical Microbiology,1996, 14th Edition, Churchill Livingstone. 2.Adams B. A., 1929, Water and Water Eng., 31:327. 3.Eaton A. D., Clesceri L. S. and Greenberg A. W., (Eds.), 2005, Standard Methods for the Examination of Water andWastewater, 21st Ed., APHA, Washington, D.C. 4.Lechevalier H. A., 1975, Environ. Protection Technol. Ser., EPA-600/ 2-75-031, U. S. Environmental Protection Agency,Cincinnati, Ohio. 5.Lechevalier M. P., and Lechevalier H. A., 1974, Int. J. Syst.Bacteriol., 24:278. 6.Pine L., and Watson S. J., 1959, J. Lab. Clin. Med., 54(1), 10 |
| Customized Product Available | No |
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