HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Recommended for transportation of Neisseria species and other fastidious organisms from clinic to laboratory.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Calcium chloride | 0.100 |
| Sodium glycerophosphate | 10.000 |
| Sodium thioglycollate | 1.000 |
| Methylene blue | 0.002 |
| Agar | 3.000 |
| Final pH (at 25°C) | 7.4±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Using the capped swab, provided along with the media containing tube, collect the sample to be transported.Discard the cap of the tube and insert the capped swab with the sample till the bottom of the medium. Tighten the cap firmly. The specimen will be preserved during transportation and also the viability of the organisms will be maintained but it will diminish over the time. Some growth of contaminants may occur during longer period of transport. After the transportation, the specimen should be inoculated in proper medium as soon as possible. The cultures on transport swabs must not be kept at room temperature for more than 24 hours.
Principle And Interpretation
Stuart transport medium was designed by Stuart for Gonococci (3). The medium is chemically defined, semisolid, non-nutrient medium which will prevent microbial proliferation. Because of composition of medium microorganisms are able to survive for a sufficiently long period. The medium provides sufficient anaerobiosis which is monitored by means of the redox indicator methylene blue. Calcium chloride and glycerophosphate provide a good buffering capacity to the medium and also maintains osmotic equilibrium in the medium. Sterile cotton swabs allow absorption of specimen material while polylpropylene shaft allows semiflexibility to the swab stick, aiding in collection. Stuart transport medium was designed by Stuart for Gonococci (3). The medium is chemically defined, semisolid, non-nutrient medium which will prevent microbial proliferation. Because of composition of medium microorganisms are able to survive for a sufficiently long period. The medium provides sufficient anaerobiosis which is monitored by means of the redox indicator methylene blue. Calcium chloride and glycerophosphate provide a good buffering capacity to the medium and also maintains osmotic equilibrium in the medium. Sterile cotton swabs allow absorption of specimen material while polylpropylene shaft allows semiflexibility to the swab stick, aiding in collection.
Type of specimen
Clinical samples - Gonococcal specimens.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (1,2).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. Read the label before opening the container. Wear protective gloves/protective clothing/ eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further recovery from this enriched medium onto selective media is required.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Stuart Transport Medium in tubes with sterile viscous swabs
Colour
Whitish to light blue coloured medium
Quantity of Medium
8ml of medium in tubes
Sterility test
Passes release criteria
Reaction
7.20-7.60
Cultural response
Viability of following organisms was established for a period of 48 hours. Organisms grew luxuriantly when inoculated on Chocolate Agar (M103) and incubated at 35 - 37°C for 18-24 hours.
| Organism | Recovery |
|---|---|
| Neisseria gonorrhoeae ATCC 19424 | Luxuriant |
| Streptococcus pneumoniae ATCC 6303 | Luxuriant |
| Haemophilus influenzae ATCC 35056 | Luxuriant |
Storage and Shelf Life
Store between 5-25°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | HiCulture™ Transport Swabs w/ Stuart Transport Medium |
|---|---|
| SKU | MS306 |
| Customized Product Available | No |
Write Your Own Review