HiMedia Laboratories GmbH, Marie-Curie-Str. 3, 64683, Einhausen, Germany.
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Intended use
Recommended for primary culturing and transport of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. Combination of solid (7 ml) and liquid (20 ml) media in single bottle.
Composition**
Proprietary
Directions
- Mark the dual performance bottle for sample detail.
- Add 3-5 ml of sample suspension to the broth medium by opening bottle under aseptic conditions and after addition, replace the cap. Incubate at 37°C for 4-5 hour.
- After 4-5 hours of incubation remove the bottle from incubator. Tilt the bottle horizontally whereby solid medium is submerged with liquid phase. Keep it for 30-40 seconds and again bring bottle to standing position. Transfer bottle to incubator and incubate further at 37°C for 18 to 20 hours. After incubation read the results as indicated.
Principle And Interpretation
This is diphasic medium developed for the transport of the primary cultures of cerebrospinal fluids from patients with bacterial meningitis. It contains nutrients such as charcoal, starch, and gelatin which support the growth of fastidious organisms like N. meningitidis, S. pneumoniae, H. influenzae. Activated charcoal and starch neutralizes different inhibitory substances acting as detoxifying agents. Gelatin in the medium preserves bacteria by forming a protective coat on the cell surface.
Type of specimen
Clinical samples : cerebrospinal fluid
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (1,2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological testing is required for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
In a sterile glass bottle combination of broth and one agar coated surface.
Colour of Agar medium
Black coloured media
Colour of liquid medium
Yellow coloured solution
Quantity of medium
7ml of agar media in glass bottle and 20ml of liquid medium in glass bottle
pH of Agar medium
7.20-7.40
pH of liquid medium
7.20-7.40
Sterility Check
Passes release criteria
Cultural response
Cultural characteristics was observed after incubation at 35-37°C at 5-10% CO2 for 24 - 48 hours.
| Organism | Inoculum (CFU) | Growth on agar medium | Growth on liquid medium |
|---|---|---|---|
| Neisseria meningitidis ATCC 13090 | 50-100 | Good-luxuriant | Luxuriant |
| Haemophilus influenzae ATCC 19418 | 50-100 | Good-luxuriant | Luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | Good-luxuriant | Luxuriant |
| Neisseria gonorrhoeae ATCC 19424 | 50-100 | Good-luxuriant | Luxuriant |
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | HiCombi™ Dual Performance Trans Isolate Medium |
|---|---|
| SKU | LQ109 |
| Customized Product Available | No |
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