Fluid Thioglycollate Medium w/ 0.05% SPS

Principle And Interpretation

Brewer (1) formulated Fluid Thioglycollate Medium for rapid cultivation of aerobes as well as anaerobes including microaerophiles by adding a reducing agent and small amount of agar. The USP (2), BP (3), EP (4) and AOAC (5) have recommended the media for sterility testing of antibiotics, biologicals and foods and for determining the phenol coefficient and sporicidal effect of disinfectants. However, it is intended for the examination of clear liquid or water-soluble materials. Fluid Thioglycollate Medium is also routinely used to check the sterility of stored blood in blood banks (6). Dextrose, tryptone, yeast extract, L-cystine provide the growth factors necessary for bacterial multiplication. L cystine and sodium thioglycollate allows Clostridium to grow in this medium even under aerobic conditions (7). Also the small amount of agar used in the medium favors the growth of aerobes as well as anaerobes in the medium, even if sodium thioglycollate is deleted from the medium (1). Sodium thioglycollate act as a reducing agent and neutralizes the toxic effects of mercurial preservatives and peroxides formed in the medium, thereby promoting anaerobiosis, and making the medium suitable to test materials containing heavy metals. (8,9). Any increase in the oxygen content is indicated by a colour change of redox indicator, resazurin to red (10). The small amount of agar helps in maintaining low redox potential for stabilizing the medium (9). SPS (Sodium polyanethol sulphonate, SPS) is used as a nontoxic anticoagulant which enables bacterial growth and prevents the action of natural bacterial inhibitors of blood. Van Haebler and Miles (11) first demonstrated the usefulness of SPS in Blood culture medium. Note: If more than upper one-third of the medium has acquired a pink-purple colour, the medium may be restored once by heating in a water bath or in free flowing steam until the pink colour disappears.