Dulbecco’s Modified Eagle Medium (DMEM)

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mg/L of glucose and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4500mg/L of glucose. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. AL294A is 2X Dulbecco's Modified Eagle Medium with 9.0gms Glucose per litre and L-Glutamine. It does not contain Sodium bicarbonate and Sodium pyruvate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines