Minimum Essential Medium Eagle (MEM) with Earle's Salts, L-Glutamine, 25mM HEPES, NEAA and Sodium bicarbonate

Minimum Essential Medium(MEM) is a modification of Basal Medium Eagle(BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL260A contains Earle's salt, 25mM HEPES buffer, non-essential amino acid, L-glutamine and sodium bicarbonate.