Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for selective isolation and cultivation of fungi.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™™ peptone | 10.000 |
| Dextrose (Glucose) | 20.000 |
| Chloramphenicol | 50mg |
| Cycloheximide | 500mg |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 45.54 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Sabouraud Dextrose Agar was originally formulated by Sabouraud (1) and further modified by Emmons (2) by reducing dextrose content and adjusting the pH close to neutral. Sabouraud Cycloheximide Chloramphenicol HiVeg™™ Agar is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™™ peptone is the source of nitrogenous growth factors while dextrose provides an energy source for the growth of microorganisms. The media can be rendered selective for fungi by antibiotics such as Chloramphenicol (3) and Cycloheximide (4), which inhibit some bacteria as well as some saprophytic and pathogenic fungi. This medium inhibits fungi like Cryptococcus neoformans, Aspergillus, Nocardia, certain Candida species but allow the dermatophytes to grow well.
Type of specimen
Please add specimens
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Some pathogenic fungi may produce infective spores, which are easily dispersed in air, so examination should be carried out in safety cabinet.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of Prepared Medium
Light amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.5% w/v aqueous solution at 25°C. pH: 6.8±0.2
pH
6.60-7.00
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 2-3 weeks.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| *Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | none-poor | |
| Candida albicans ATCC 10231 (00054*) | 50-100 | poor-fair | <=20% |
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | 0% |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | none-poor | <=20% |
| Trichophyton mentagrophytes ATCC 9533 | 50-100 | luxuriant | |
| Trichophyton rubrum ATCC 28191 | 50-100 | luxuriant |
Key: (*) - Corresponding WDCM numbers. (#) - Formerly known as Aspergillus niger
Storage and Shelf Life
Store between dehydrated and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Sabouraud Cycloheximide Chloramphenicol HiVeg™ Agar |
|---|---|
| SKU | MV664 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Sabouraud R., 1892, Ann. Dermatol. Syphilol., 3:1061. 2.Emmons C., Binford C., Uty J. and Kwon-Chung, 1970, Medical Mycology, 2nd ed., Philadelphia: Lea and Febiger. 3.Diagnostic Procedures, 1963, 4th ed., APHA4 Ajello L., 1957, J. Chron. Dis., 5:545.,,5 MacFaddin J. F., 1985, Media For Isolation-Cultivation Identification - Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore. ,, |
| Customized Product Available | No |
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