Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
SCHWARZ Differential HiVeg Medium is used in the brewing industry for the differentiation of brewing yeasts from wild yeasts.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 5.00 |
| Yeast extract | 3.00 |
| Malt extract | 3.00 |
| Dextrose | 10.00 |
| Basic fuchsin | 0.47 |
| Sodium sulphite | 2.92 |
| Dextrin | 0.11 |
| Agar | 20.00 |
Final pH (at 25°C) 6.9 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Product Profile
Vegetable based (Code MV): MV1331 HiVeg peptone
Animal based (Code M): M1331 Peptic digest of animal tissue
Recommended for: Differentiation of brewing yeasts from wild yeasts.
Reconstitution: 44.50 g/l
Quantity on preparation (500g): 11.23 L
pH (25°C): 6.9 ± 0.2
Supplement: None
Sterilization: Boiling (DO NOT AUTOCLAVE)
Storage: Dry Medium Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 44.50 grams in 1000 ml distilled water. Heat to boiling with constant stirring for 15 minutes. DO NOT AUTOCLAVE. Cool to 45°C and pour into sterile plates. Efficacy of the plates can be improved by incubating them at 30°C for 18 hours before use.
Caution: Basic fuchsin is a potential carcinogen and care should be taken to avoid inhalation of the powdered dye and contamination of the skin.
Principle and Interpretation
SCHWARZ Differential HiVeg Medium is prepared by using HiVeg peptone which is free of BSE/TSE risks associated with animal based peptones. This is the modification of SCHWARZ Differential Medium which is recommended for use in the brewing industry for the differentiation of brewing yeasts from wild yeasts. HiVeg peptone and yeast extract provides necessary nutrients to support the growth of yeasts. Malt extract, dextrose serves as suitable carbon source for the growth of yeasts. Also dextrin, a complex carbon source is utilized by few brewing yeasts, like Saccharomyces diasticus. Sodium sulphite and basic fuchsin inhibits most gram-positive bacteria. Acid producing bacteria are identified by the depth of a clear zone around the colonies. Actidione may be added to the medium to suppress growth of wild culture yeast. The prepared plates darken during incubation. Wild yeasts form pink colonies which may be smooth, mucoid or wrinkled. Brewing yeasts forms a thin haze of micro colonies which blend with the colour of the medium.
Quality Control
Appearance of Powder: Orangish pink coloured, homogeneous, free flowing powder.
Gelling: Firm, comparable with 2.0% Agar gel.
Colour and Clarity: Light pink coloured, clear gel forms in petri plates.
Reaction: Reaction of 4.45% w/v aqueous solution is pH 6.9 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 30°C upto 4 days (colour of the plates darkens during incubation).
| Organisms (ATCC) | Growth | Colour of Colony |
|---|---|---|
| Candida albicans (10231) | luxuriant | white to light pink raised colonies |
| Candida krusei (24408) | luxuriant | pink, rough, flat colonies |
| Saccharomyces cerevisiae (9763) | luxuriant | pink colonies |
| Product Name | SCHWARZ Differential HiVeg™ Medium |
|---|---|
| SKU | MV1331 |
| Customized Product Available | No |
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