IgG Purification Kit (Protein A Based, Spin Column)

IgG Purification Kit (Protein A Based, Spin Column) is based on the affinity of IgG to immobilized Protein A agarose and is used for purification of polyclonal antibodies.Protein A contains five homologous antibody–binding domains with high affinity for subclasses of IgG from many species and for some IgA and IgM. it is used for the Purification of IgG fractions (from crude serum, ascites fluid, tissue culture supernatant) and for Isolation of antigen-antibody complex (during immunoprecipitation) based on spin column technique utilizing centrifugal force. The kit contains pre-packed ready-to-use columns of 0.5ml Protein A agarose resin in 20% ethanol with a binding capacity of 25mg human IgG /ml of resin.