Hi-PCR® Salmonella Food Detection Kit (Provided with culture media)

Salmonellosis is a major public health concern worldwide resulting in thousands of deaths. This food‐borne disease is caused by Salmonella, a rod shaped, gram‐negative non‐spore forming bacterium, a member of Enterobacteriaceae family. The mode of infection is contaminated food. The traditional culture‐based detection takes longer for detection of Salmonella, hence, the need for new, quick and sensitive methods to detect Salmonella spp. is a major concern for the food industry.

NOTE: The Salmonella Food Detection Kit is for in‐vitro use only.

Intended Use

This kit is designed for detection of specific sequence of iroB (606 bp) gene for Salmonella spp. from various food samples. PCR testing can provide rapid, sensitive and specific detection of Salmonella spp. This kit also contains Internal control and Positive control.

Principle

HiMedia's Salmonella Food Detection Kit is a Semi‐Quantitative PCR Kit which contains the amplification of Salmonella spp. specific gene using specific primers. The amplified target is detected by using agarose gel electrophoresis.

Positive control

This is a control reaction using a known template (target pathogen). A positive control is usually used to check that the primers have been designed properly and the PCR conditions have been set up correctly.

Internal control

This is a control sequence amplified in the same reaction tube along with the target sequence (target pathogen) but detected with a different primer (i.e. Multiplex PCR). An internal control is often used to detect the failure of amplification in cases where the target sequence is not amplified PCR is an efficient, specific way to amplify the desired small segments of the genetic material. The three steps of a PCR reaction are Denaturation, Annealing and Extension. During the Denaturation step, the double stranded DNA gets converted to single stranded DNA due to high temperatures. In the Annealing step, a set of primers specific to the genetic sequence bind the single stranded DNA. In the Extension step, the dNTPs are incorporated by the Taq Polymerase to create a new strand of the DNA which is complimentary to each of the single template strands. Gel electrophoresis is used to analyse the amplification of desired gene region for target pathogen based on separation of DNA fragments according to their size.

Features

• Fast and simple
• Sensitive and specific results
• Guaranteed reproducible results