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HiPurA™ Yeast RNA Purification Kit

Packings :
MB611-250PR | MB611-50PR
• Yeast cells are difficult to lyse as they are normally protected by a thick cell wall which makes extraction of cellular RNA difficult. This kit simplifies isolation of yeast RNA with spin-column procedure. • Enzymatic digestion of the cell wall with zymolyase/Lyticase, helps spheroplasts formation that renders the cells vulner able to easy lysis with detergents or rapid osmolar pressure changes. • Once the spheroplast is formed, lysis buffer provided in the kit helps in cell disruption and denaturation. Samples are then passed through a HiShredder which removes insoluble material and reduces the viscosity of the lysate by disrupting viscous material. • Ethanol is added to the cleared lysate, which promotes selective binding of RNA to the silica membrane. • After the initial binding of RNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality RNA is finally eluted in the elution solution provided with the kit.
Used For
The RNA obtained is compatible with the following downstream applications • RT-PCR • Cloning purpose • cDNA library construction
Technical Data
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