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Antibody Capture ELISA Teaching Kit

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Enzyme linked immunosorbent assay or ELISA is a sensitive immunological technique to detect the presence of a specific antigen (Ag) or antibody (Ab) in a biological sample. It utilizes the dual properties of antibody molecules being specific in reactivity and their ability to be conjugated to active molecules such as enzymes. An enzyme conjugated with an antibody reacts with a chromogenic colorless substrate to generate a coloured reaction product. ELISA is extensively used for diagnostic purpose which utilizes the dual. It requires an immobilized antigen/antibody bound to a solid support (e.g. microtitre plate or membrane).
There are different types of ELISAs for the detection of a protein of interest in a given sample. One of them is Antibody Capture ELISA which can measure the concentration of an antibody through an indirect ELISA method. This method is very useful for the screening of anti serum for specific antibodies.
Used For
Antibody Capture ELISA Teaching Kit enables the quantitative detection of antibody bound to the immobilized antigen using a labelled secondary antibody.
Contents: Coating Antigen, Standard Primary Antibody, Test Samples, HRP-Conjugated Secondary Antibody, Blocking Buffer, TMB/H2O2, Coating Buffer, PBST, Stop Solution, Microtitre Plate, Stop Solution
Technical Data
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