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Medium 199
w/ Earle’s Salts, L-Glutamine and 25mM HEPES buffer
w/o Sodium bicarbonate

Packings :
Medium 199 was the first nutritionally defined medium developed by Morgan, Morton and Parker in 1950. This complex medium was formulated specifically for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. It was obser
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