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Most cell culture media utilize the CO2-bicarbonate buffering system to maintain the pH since it is less toxic, cheap and has certain nutritional value. The growth and metabolism of cultured mammalian cells are markedly affected by the pH variation in ordianary bicarbonate buffered media. These pH swings can be reduced and the pH of the culture can be stabilized as desired by using appropriate buffers.
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HEPES 1M Solution
Cell culture tested

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