Hi-PCR® White Spot Syndrome Virus (WSSV) Detection Kit (Extraction and Semi-Q PCR Combo Kit)

This kit simplifies isolation of DNA from shrimp tissue samples with spin‐column based procedure. Shrimp tissue (spliced and digested) is subjected to lysis by Proteinase K in a chaotropic salt solution. Following lysis, is the binding of DNA to the silica membrane of the HiEluteMiniprep Spin Column to yield purified DNA. Rapid wash steps remove trace salt and protein contaminants resulting in the elution of high quality DNA in the Elution Buffer (ET) provided with the kit. The WSSV PCR Kit is a qualitative conventional PCR kit which includes the amplification of specific gene regions of 146 F2/146 R2 (amplicon size: 941 bp), an insertion element found exclusively within the members of the Shrimp samples using specific primer. The amplified target is confirmed by using agarose gel electrophoresis. This kit contains an Internal and Positive control.