Hi‐PCR® Anthrax (Multiplex) Probe PCR Kit is designed to detect a genetic marker 5345 and two virulence geneslocated on plasmid pX01 and pX02 of Bacillus anthracis in FAM, ROX and Cy5 channel, respectively, and the Internal Control in JOE channel in a single tube reaction. The kit allows sensitive and specific detection of Bacillus anthracis.
Bacillus anthracis (B. anthracis), the causative organism of Anthrax is a Gram?positive spore forming, non? motile bacillus commonly found as endospores in soil. Anthrax is a zoonotic disease which is mainly associated with herbivores and domestic animals. Human anthrax is less common and usually spreads to human populations through close occupational proximity to infected livestock by handling infected domestic animals including cattle and goats or their products like skin, meat, hides and bones. The potential use of this bacterium as a bioterrorism agent haslong been suspected because itsspores can be aerosolized and sprayed to spread disease, are extremely resistant to natural conditions and can survive for several decades in the environment.