Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Brent Supplement Mixture (BSM) is recommended for use with the addition in DOB or DOBA and carbon source for the growth of Saccharomyces cerevisiae.
Composition
| Ingredients | Milligrams/Litre |
|---|---|
| Adenine | 40.00 |
| L-Arginine HCI | 20.00 |
| L-Aspartic acid | 100.00 |
| L-Glutamic acid | 100.00 |
| L-Histidine HCl | 20.00 |
| L-Isoleucine | 300.00 |
| L-Leucine | 60.00 |
| L-Lysine HCl | 30.00 |
| L-Methionine | 20.00 |
| Para-aminobenzoic acid (PABA) | 8.6 |
| L-Phenylalanine | 50.00 |
| L-Serine | 375.00 |
| L-Threonine | 200.00 |
| L-Tryptophan | 40.00 |
| L-Tyrosine | 30.00 |
| Uracil | 20.00 |
| L-Valine | 150.00 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 1.55 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
Brent Supplement Mixture (BSM) is recommended for use with the addition in DOB (Dropout Base) or DOBA (Dropout Based Agar) and carbon source for the growth of Saccharomyces cerevisiae. This yeast strain is called budding yeast and is extensively studied microorganism in molecular and cell biology. Brent Supplement Mixture (BSM) is used as an amino acid supplement for all strains of Saccharomyces cerevisiae which supplies the essential nutritional elements for budding yeast cells. It is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae. It can be mixed with yeast nitrogen base (YNB), Ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose) to produce a media suitable for the growth of budding yeast.
Quality Control
Appearance of Powder : White to cream coloured, homogeneous, free flowing powder.
Colour and Clarity : Colourless, clear solution without any precipitate.
Cultural Response
Cultural characteristics observed after incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC 9763 | good-luxuriant |
Storage and Shelf-life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Brent Supplement Mixture (BSM) |
|---|---|
| SKU | G101 |
| Customized Product Available | No |
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