Experience Extraction with HiMedia

Want to overcome the obstacles of nucleic acid extraction?
Experience Easy Extraction with HiMedia

What is plant research?
Plant is one of the major sources which fulfill our basic needs. Anything we eat or the clothes we wear is derived either directly or indirectly from plant sources. This becomes the obvious and one of the most important reasons to dwell more into plant research areas with respect to their gene sequence and gene function, for which nucleic acid extraction is very crucial and mandatory step.

Why plant research is required?
Human Health : One of the pressing needs of the current scenario, is to improve human health which can only be achieved by improving the nutritional content of crops, which is possible by genetically engineered plants.
Crop Improvement : Plant genomics has opened new ways and options for plant breeder to introduce beneficial genes to produce transgenic plants which are resistant to pest and other abiotic stresses.
Functional Genomics : Whole genome sequencing is a boon to providing evidence about evolutionary journey of a functional gene as well as in recognizing an indigenous species.
Medicinal Plants and Research : Natural products of medicinal plants are significant source for phytodrugs because of their therapeutic properties such as, antifungal, antibacterial, antimalaric, contraceptive, antipyretic, anti-inflammatory, analgesic, anti-hypertensive, antioxidant, anticancerogenic etc. Hence vast research on genetic component of these medicinal plants is being carried out.

What is nucleic acid extraction?
In nucleic acid extraction, DNA/RNA is isolated from proteins and other cell components. It is routine practice in clinical field, food industry, forensic science as well as plant and soil testing.

The procedure usually follows three main basic steps:
1. Dissociation of tissue
2. Cell lysis to release DNA and,
3. Purification of DNA

Experimenting with an IMPURE sample can give you erroneous results. In order to avoid such happenings, it is important to ensure purity of nucleic acid, which then can be used for PCR, sequencing etc.
Purity of nucleic acids can be checked by quantifying the samples with spectrophotometer. Getting a value of approximately 1.6 to 1.8 at A260/ A280 ensures purity of DNA. However, pure RNA has an A260/A280 ratio of approximately 1.8 to 2.0.

Problems Associated with Plant DNA Extraction
Earlier, nucleic acid extraction used to be labor intensive and time consuming process. Apart from that, it is crucial to obtain a good yield of the purified extract. Moreover, some species of plants (like banana, pomegranate, sugarcane etc.) contain phenolic compounds and plants like cotton contains high levels of polysaccharides, oxidizable quinones which makes DNA extraction a troublesome process. Presence of these organic compounds tremendously inhibit the process of DNA extraction.

Here We Need to Just Pick up the Right Method

A Correct Choice is not Just a Solution, it is the Elimination of the Problem
Because of vast diversity in plant species, it is very difficult to follow single protocol for all. HiMedia with extensive research and optimization has developed innovative column based extraction kits to give very high yield of up to 500ng/μL and purity ratio of 1.8 to 2.0. For example HiMedia's HiPura™

SuperPlant DNA Purification Kit, a column based protocol is the best alternative to manual methods and wellsuited to isolate DNA from all the plants which contain phenolic compounds and diminish the risk of using harmful chemicals to obtain high yield.