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Sandwich ELISA Teaching Kit

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Enzyme linked immunosorbent assay or ELISA is a sensitive immunological technique to detect the presence of a specific antigen (Ag) or antibody (Ab) in a biological sample. It utilizes the dual properties of antibody molecules being specific in reactivity and their ability to be conjugated to active molecules such as enzymes. An enzyme conjugated with an antibody reacts with a chromogenic colourless substrate to generate a coloured reaction product. ELISA is extensively used for diagnostic purpose, which utilizes the dual. It requires an immobilized antigen/antibody bound to a solid support (e.g. microtitre plate or membrane).
There are different types of ELISAs for the detection of a protein of interest in a given sample. One of the most common ELISA is Sandwich ELISA which can measure the amount of antigen which is sandwiched between two layers of antibodies i.e. capture and detection antibody.
Used For
Sandwich ELISA Teaching Kit enables the determination of antigen concentration bound to immobilized antibody followed by binding of the antigen to the labelled secondary antibody and its detection by using appropriate substrate.
Contents: Standard Antigen, Capture Antibody, Test Samples, HRP-Conjugated Antibody, Blocking Buffer, Coating Buffer, TMB/H2O2, PBST, Stop Solution, Microtitre Plate
Technical Data
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