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HiPurA™ Total RNA Miniprep Purification Kit

Packings :
MB602-20PR | MB602-50PR
MB602-250PR |
• HiPurA™ Total RNA Miniprep Purification Kit can be used for purification of total RNA from animal cells, animal tissues, bacteria and yeast, and for cleanup of RNA from crude RNA preparations and enzymatic reactions with spin-column procedure. • The lysis buffer provided in the kit helps in cell disruption and denaturation; samples are centrifuged through a HiShredder which removes insoluble material and reduces the viscosity of the lysate by disrupting viscous material. • Ethanol is added to the cleared lysate, which promotes selective binding of RNA to the silica membrane. After the initial binding of RNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality RNA is finally eluted in the elution buffer provided with the kit.
Used For
The RNA obtained is compatible with the following downstream applications • Northern analysis • Poly A+ RNA selection • Primer extension • RNase and S1 nuclease protection assays • RT-PCR, Differential display • Expression-array and expression-chip analysis • cDNA library construction
Technical Data
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