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HiPurA™ Plant and Fungal RNA Miniprep Purification Kit

Packings :
MB603-20PR | MB603-50PR
MB603-250PR |
• This kit simplifies isolation of RNA from plant cells, tissues and filamentous fungi with spin-column procedure. • The RNA - XPress Reagent provided in the kit helps in cell disruption and denaturation of the plant material. • After adding chloroform and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing cell debris and DNA and an organic phase containing proteins. • To the aqueous phase, Binding Solution and ethanol are added, which promote selective binding of RNA to the silica membrane. • After the initial binding of RNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality RNA is finally eluted in the Elution Solution.
Used For
The RNA obtained is compatible with the following downstream applications • Northern analysis • Poly A+ RNA selection • Primer extension • RNase and S1nuclease protection assays • RT-PCR • Differential display • Expression-array and expression-chip analysis • cDNA library construction • RNase and S1 nuclease protection assay
Technical Data
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