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HTBM027

Southern Blotting Teaching Kit

 
Packings :
HTBM027-5PR
Description
Southern blotting or Southern hybridization is a widely used technique in molecular biology for transfer of DNA molecules; usually restriction fragments, from an electrophoresis gel to a nitrocellulose or nylon membrane, and is carried out prior to detection of specific molecules by hybridization probing. In this method a DNA mixture is separated by agarose gel electrophoresis according to their size followed by transfer of the DNA bands to nitrocellulose/nylon membrane. Finally, the DNA of interest is probed for a specific sequence.
Used For
Southern Blotting Teaching Kit demonstrates the process of separation of DNA bands on agarose gel followed by transfer of DNA from gel to membrane and detection of specific DNA bands by a chromogenic method.
Contents: DNA sample, Prehybridization and Hybridization Buffer, Biotinylated Probe, Wash Buffer, Blocking Buffer and Powder, Streptavidin-HRP Conjugate, Tween 20, Agarose, 10X TBE, Transfer Buffer, Nylon Membrane with Filter Paper, Disposable Petriplate
 
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Technical Data
MSDS
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