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HTBM024

RT-PCR Teaching Kit

 
Packings :
HTBM024-5PR
Description
Reverse transcription polymerase chain reaction or RT-PCR is a modified version of PCR. This is a two step process where RNA strand is first reverse transcribed to complementary DNA (cDNA) by the enzyme reverse transcriptase and the resulting cDNA is amplified by the traditional PCR. In RT-PCR a pair of primers are used which are complementary to a defined sequence on each of the two strands of the cDNA. These primers (annealed to the cDNA) are then extended by a DNA polymerase and a copy of the strand is made after each cycle which leads to the exponential amplification. It is a very sensitive method and is used to generate large cDNA libraries from very small amount of mRNA and in gene expression studies.
Used For
RT-PCR Teaching Kit facilitates the reverse transcription of RNA to its complementary DNA using reverse transciptase enzyme which is then amplified using RT-PCR and visualization of amplified products using agarose gel electrophoresis method.
Contents: Total RNA, Random hexamer, RNase inhibitor, RT Buffer, Oligo dT, MB Grade Water, MgCl2, dNTP Mix, MMuLV Reverse Transcriptase, Agarose, 50X TAE, Loading Dye, Primers, DNA Ladders, Taq Polymerase, Tubes.
 
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Technical Data
MSDS
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