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HTBM016

PCR Teaching Kit

 
Packings :
HTBM016-10PR
Description
Polymerase Chain Reaction (PCR) is an in vitro method of enzymatic synthesis of specific DNA fragment developed by Kary Mullis in 1983. It is a very simple technique for characterizing, analyzing and synthesizing DNA from virtually any living organism (plant, animal, virus, bacteria). PCR is used to amplify a precise fragment of DNA from a complex mixture of starting material called as template DNA.
A basic PCR requires the following components:
1. DNA template that contains the region to be amplified
2. Two primers complementary to the 3’ ends of each of the sense and anti-sense strand of the DNA
3. Thermostable DNA polymerase like Taq, Vent, Pfu etc.
4. Deoxynucleoside triphosphates (dATP, dCTP, dGTP and dTTP), the building blocks from which the DNA polymerase synthesizes a new DNA strand.
5. Buffer solution which provides a suitable chemical environment for optimal activity and stability of DNA polymerase.
6. Bivalent magnesium/manganese ions, which are necessary for maximum Taq polymerase activity and influences the efficiency of primer to template annealing.
Used For
PCR Teaching Kit facilitates rapid amplification of genomic DNA using Taq polymerase and visualization of the amplified DNA using agarose gel electrophoresis method.
Contents: 10X Assay Buffer, Control PCR Product, 2.5 mM dNTP mix, 1 kb DNA ladder, primers, Taq polymerase, Template DNA, MB Grade Water, MgCl2, Agarose,50X TAE, Gel Loading Buffer, Mineral Oil, PCR Tubes.
 
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